Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Rat Calvarial Tissues Response to Flowable DNA/protamine Complex Mixtures with DNA/chitosan Complex to be Used as a Protective Membrane for Guided Bone Regeneration

 Yosuke SHINOZAKI1, Nana MORI2, Jun OHNO3, Minoru KAWAGUCHI4, Hirofumi KIDO1, Tohru HAYAKAWA5 and Tadao FUKUSHIMA6
1Department of Oral Rehabilitation, Oral Implantology section,
2Department of Odontology, Periodontology section,
3Department of Morphological Biology, Pathology section,
4Department of Dental Engineering, Bioengineering section,
Fukuoka Dental College, Fukuoka, Japan
5Department of Dental Engineering, Tsurumi University, School of Dental Medicine, Yokohama, Japan
6Center for Regenerative Medicine, Fukuoka Dental College, Fukuoka, Japan

J Oral Tissue Engin 2011;9(3):159-166
SYNOPSIS
Complex mixtures of a DNA/protamine complex with 30%, 50%, and 70% DNA/chitosan complex were prepared for a soft type of membrane for guided bone regeneration. All complex mixtures showed flowable properties when kneading the mixture with distilled water. Increases in the mixture ratio of the DNA/chitosan complex in the complex mixture increased the consistency of flowable complex mixture. Differences in biodegradable rates among three complex mixtures were observed in vivo. Although the complex mixture with 30% DNA/chitosan complex completely disappeared, part of the other complex mixtures remained 3 months after implantation. Biodegradable rates of mixtures with higher volumes of the DNA/chitosan complex were slower. In conclusion, all complex mixtures showed flowable properties. Their consistency and biodegradable rates could be controlled by the mixing ratio between DNA/protamine and DNA/chitosan complexes. They are promising candidates for biodegradable membranes with adjustable biode-gradable rates that form desired figures by hand during surgery.

Key words: DNA/protamine complex, protective membrane, guided bone regeneration