Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Cell Viability and Tissue Response of High Molecule Weight DNA/protamine Complex

 Nana MORI1, Teruaki IWAHASHI2, Jun OHNO2, Yosuke SHINOZAKI3, Ryuji SAKAGAMI1, Makoto MITARAI4 and Tadao FUKUSHIMA5
 1Department of Odontology, Periodontology section, 2Department of Morphological Biology, Pathology section, 3Department of Oral Rehabilitation, Oral Implantology section, Fukuoka Dental College, Fukuoka, Japan
4Central Research Institute, Maruha nichiro holdings, Inc, Tsukuba, Japan
5 Department of Dental Engineering, Bioengineering section, Fukuoka Dental College, Fukuoka, Japan

J Oral Tissue Engin 2011;8(3): 188-194
SYNOPSIS
The DNA/protamine complex was prepared from the reaction of high molecule weight DNA and protamine. The obtained high molecule weight DNA/protamine complex was water-insoluble white powder. The yield was 75%. The molar binding ratio (molar of protamine/ 1 base pair of DNA) DNA to protamine in was 0.129. SEM observation revealed that prepared complex had a porous structure. The porous disk could be easily prepared. In cell culture tests using MC 3T3-E1 mouse osteoblast cells, cell viabilities for the DNA/protamine complex were from 87.1% to 99.0%. In vivo test, complex showed a mild tissue response after the implantation into the back of skin of rats. The complex was gradually biodegraded during implantation, and a part of complex resided 30 days after implantation. These results suggested that high molecule weight DNA do not influence cytotoxicity and tissue response of DNA/protamine complex.

Key words: high molecule weight DNA, protamine, DNA/protamine complex, Cell viability, tissue response