SYNOPSIS
In the present in vitro study, we evaluated peri-implant (IP) osteogenesis generated by 3-D culture of osteogeneic JCRB1119:KUSA/A1 cells with a collagen gel scaffold on Ti and Ti alloy IPs, which have osteoconductive and osteoinductive surface texture and substrates (i.e., IP-AO, IP-HA, IP-SPI) that are commercially available. In the histological study, we identified bone elaboration associated with formation of cement line (interfacial matrix) on the different substrate surfaces; the integrated mineralizing layer provided a biomimetic scaffold which favored static osteogenesis at the IP surfaces. Meanwhile, we elucidated contact osteogenesis showing different distribution and morphology at the cell-substratum interface. Furthermore, matrix mineralization showing the histology that mimicked distant osteogenesis in vivo was evident in the growing peri-IP opaque matrix; the IP-HA have acquired more opaque matrix in the day 21 specimens. The present GBR method engineered KUSA/A1 cells in a 3-D collagen scaffold to initiate an osteogeneic process of new (de novo) bone formation without intervening soft tissue in the peri-IP osteogenesis; this peri-IP osteoid tissue might be employed to a biocompatible and biodegradable tissue, which could be remodeled and modeled for functional osseointegration.

Key words: osseointegration, titanium implant, KUSA/A1 cell, collagen scaffold, in vitro