Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Effects of Ultrasound on Bone Formation Induced by BMP-2

 Kozo YAMAJI, Maiko NISHIMURA, Yoshihiro NISHITANI, Shunji IZAWA, Tomohiro HOSHIKA, Shintaro SHIODE and Masahiro YOSHIYAMA

 Department of Operative Dentistry, Field of Study of Biofunctional Recovery and Reconstruction,
Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

J Oral Tissue Engin 2010;8(1): 47-51
SYNOPSIS
Low-intensity pulsed ultrasound (LIPUS) is therapeutic instrument of improving bone healing, and Bone Morphogenetic Protein-2 (BMP-2) has been demonstrated to enhance bone formation. The present study was designed to evaluate the effects of LIPUS on bone formation induced by BMP-2 at palatal subperiosteal sites in Wister rats. Alginated gel (AG) contained BMP-2 (1.0µg/µL) was implanted into the palatal left site (BMP site), and AG alone was the right site (AG site). The rats were divided into two groups; LIPUS group, ultrasound (1MHz, 160mW, 5 min) was performed every three days, and non-LIPUS group. At 3 weeks after surgery, thickness of new bone of BMP site was significantly higher than that of AG site (P<0.05). Newly formed bone of BMP site was continuous with the original bone at non-LIPUS group, whereas it was almost integrated with the original bone at LIPUS group.
These results suggest that LIPUS has the ability to enhance the maturation of newly formed bone induced by BMP-2.

Key words: ultrasound, BMP, bone formation