Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 TEM/EDX Observation of Micro-beads of Magnetically Activated Cell Sorting (MACS) System and Separation Trial of Mouse Mesenchymal Stem Cells from Bone Marrow Cells by MACS System

 Masayuki TAIRA1, Kaori SASAKI1, Setsuo SAITOH1, Takashi NEZU1, Tadayoshi KAGIYA2, Hidemitsu HARADA2, Minoru SASAKI3, Shigenobu KIMURA3, Isao HIRATA4 and Yoshima ARAKI1

1Department of Dental Materials Science and Technology, 2Department of Oral Anatomy II and 3Department of Oral Microbiology, Iwate Medical University School of Dentistry, Iwate, Japan.
4Department of Biomaterials Science, Graduate School of Biomaterials Science, Hiroshima University, Hiroshima, Japan.

J Oral Tissue Engin 2008;6(1): 33-40
Full Text. DOI https://doi.org/10.11223/jarde.6.33
SYNOPSIS
Magnetically activated cell sorting (MACS) system is one isolation technique of mesenchymal stem cells (MSC) from bone marrow cells. TEM/EDX observation revealed that its micro-beads had the size of about 0.5 to 1.0 µm and consisted of matrix and inorganic phases. The inorganic phase contained small particles of about 40 nm size, and consisted of Fe, Si, O, Cl (e.g., Fe3O4 + SiO2 + FeCl3). In MACS system, lineage (hematopoietic) cell depletion kit reduced the cell number of bone marrow cells to 1.2 %. Subsequent positive selection by CD117, anti-Sca-1, CD34 and CD44 micro-beads lead to the cell collection rates of 0.57 %, 0.10 %, 0.13 % and 0.14 %, respectively. A combination of three micro-beads for lineage cell depletion kit, CD117 and anti-Sca-1 micro-beads succeeded in reducing the cell collection rate down to 0.04 %, matching a normal existing rate of MSC in bone marrow cells (0.01 to 0.10 %).

Key words: Mesenchymal stem cells, Magnetic cell sort, Micro-beads, Bone marrow, TEM/EDX