Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Application of Laser Capture Microdissection to Periodontal Tissue

 Yoshiko YOKOYAMA1, Teerasak DAMRONGRUNGRUANG6, Michiko SUZUKI1, Yukiko NAKANO7, Yoshiro TAKANO3, Miyuki AZUMA4, Masanaga YAMAWAKI5, Hidehiro MIZUSAWA5, Shinji KURODA1, Keiichi OHYA2, Shohei KASUGAI1 and Hisatomo KONDO1

1Section of Oral Implantology and Regenerative Dental Medicine,
2Section of Pharmacology, Department of Maxillofacial Biology,
3Section of Biostructural Science, Department of Hard Tissue Engineering,
4Department of Molecular Immunology,
5Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University, Japan
6Department of Oral Diagnosis, Faculty of Dentistry, Khonkaen University, Thailand
7Faculty of Dentistry, McGill University, Canada
J Oral Tissue Engin 2007;4(3): 155-160
Full Text. DOI https://doi.org/10.11223/jarde.4.155
SYNOPSIS
The features of cementum and periodontal ligament (PDL) remain poorly defined and a specific marker for cementum or PDL has not been identified. We therefore aimed to isolate RNA from cementoblasts and PDL cells of mouse mandibular molars using laser capture microdissection (LCM). Murine mandibular frozen sections were prepared using Tape Transfer System. Over 500 cementoblasts and PDL cells were separately captured.The quality of total RNA extracted from specimens and isolated RNAs was determined using an Agilent bioanalyzer, which detected 18s and 28s peaks in the dissected tissue, suggesting good RNA preservation. We then amplified the RNA, synthesized antisense RNA and performed RT-PCR analyses. The mRNA expression of GAPDH and bone-associated mark-ers was detected. These results suggest that LCM is useful for comparing mRNA expression in adjacent tissues.

Key words: laser capture microdissection, cementoblast, periodontal ligament