Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
Preparation of Highly Pore-interconnected Porous Collagen Sponges using Chloroform and Ammonia

Masayuki TAIRA, Kaori SASAKI, Setsuo SAITOH, Takashi NEZU and Yoshima ARAKI

Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, JAPAN


J Oral Tissue Engin 2006;4(2): 68-76

Full Text. DOI https://doi.org/10.11223/jarde.4.68

SYNOPSIS
Collagen sponges are widely used in tissue engineering. Their pore size and pore-interconnection are difficult to control. The purpose of this study was to prepare highly pore-interconnected porous collagen sponges by new pore-forming method. The type I collagen liquid (0.5wt% in HCl solution, pH 3.1) was mixed with chloroform, bubbled by blade-cutter homogenization, gelled by ambient ammonia, freeze-dried and cross-linked by dehydration in vacuum oven at 140oC for six hours. The resultant collagen sponges had the surface brick layer, but sub-surface bulk area about 2mm thick consisting of many well-interconnected homogeneous pores more than 100 mm in size. It was speculated that two porogens such as chloroform and ammonia provided the collagen sponges with well inter-connected pores during freeze-drying. Osteoblast UMR106 cells successfully adhered and multiplied on cut surfaces of prepared collagen sponges. The newly prepared highly pore-interconnected porous collagen sponges might appear to be useful for future oral tissue engineering therapy.

Key words:Collagen sponge, Inter-connected pore, Freeze-drying