Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
Monitoring of DNA Complex Formation by Means of Quartz Crystal Microbalance

Tohru HAYAKAWA1, Tadao FUKUSHIMA2, Minoru KAWAGUCHI2, Yusuke INOUE3, Kunihisa TANIGUCHI4 and Hidenori KAMINISHI5

1Department of Dental Biomaterials, Nihon University, School of Dentistry at Matsudo, JAPAN, 2Department of Dental Engineering, Bioengineering Section, Fukuoka Dental College, JAPAN, 3Fukuoka College of Health Sciences, JAPAN, 4Department of Morphological Biology, Section of Pathology, Fukuoka Dental College, JAPAN, 5Department of Functional Bioscience, Section of Infection Biology, Fukuoka Dental College, JAPAN


J Oral Tissue Engin 2006;4(2): 61-67

Full Text. DOI https://doi.org/10.11223/jarde.4.61

SYNOPSIS
The double-helical structure of double-stranded DNA enables it to form complexes with antibiotics and cytokines. The present study investigated the complex forma-tion for antibiotic or cytokine towards DNA using quarts-crystal microbalance (QCM) technique. The resonance frequency decreases in QCM system linearly upon the increase of adsorbed mass on the QCM sensor at the nanogram level. Biotinylated DNA can easily bind avidin molecules, and DNA could be immobilized onto QCM sensor, which was sputtered with gold. The crystal resonant frequency shift was immediately measured after the injection of daunorubicin hydrochloride, which was an antibiotics, and of basic fibroblast growth factor (b-FGF), which was a growth factor. The present study can monitor the complex formation between daunorubicin hydrochloride or b-FGF and DNA using QCM method, and binding amount of daunorubicin hydrochloride or b-FGF towards DNA can be easily esti-mated by the frequency decrease. In addition, the difference of binding mode of daunorubicin hydrochloride and b-FGF towards DNA was revealed by the fre-quency shift. The QCM method is a useful for monitoring DNA complex formation with antibiotics or cytokines.

Key words:DNA, Quartz Crystal Microbalance, intercalation, daunorubicin hydrochloride