Journal of Oral Tissue Engineering



Brief Communication
Cell Proliferation in Cultured Human Deciduous Pulp Fibroblast-like Cells Treated with a Novel Dental Hypersensitivity Inhibitor at 43°C

Sho AOKI1, Tomoharu OKAMURA2, Harumi ISONO2,
Rie IMATAKI1, Masae OKUNO1, Junichi FUJITA3,
Kazuya TOMINAGA2, and Yoko ABE1


1Department of Pediatric Dentistry, School of Dentistry, Osaka Dental University,
2Department of Oral Pathology, School of Dentistry, Osaka Dental University,
3Department of English, School of Dentistry, Osaka Dental University,
Osaka, Japan


J Oral Tissue Engin 2024; 22(1): 46-50.

SYNOPSIS
During childhood and adolescence, dentin hypersensitivity with substantial loss of tooth structure such as erosive tooth wear and enamel hypoplasia becomes a clinical challenge. When using dental desensitizing agents for the treatment of dentin hyper-sensitivity, the biocompatibility of the material with dental pulp cells and its impact on wound healing are crucial. Dental desensitizing agents primarily composed of calcium carbonate exhibit biocompatibility with human deciduous pulp fibroblast-like cells. It is known that human deciduous pulp fibroblast-like cells can enhance cell proliferation ability under appropriate thermal stimulation. In an environment heated to 43°C for 15 minutes, heat shock proteins and after 24 hours, the proliferation of human deciduous pulp fibroblast-like cells is enhanced. In this study, the relationship between biocom-patible dental desensitizing agents and thermal stimulation was investigated using human deciduous pulp fibroblast-like cells, revealing that cells cultured with the added material exhibited enhanced cell proliferation under conditions of thermal stimulation.

Key words: heat stimulation, 43°C, dental hypersensitivity suppressing material, cell culture, human deciduous dental pulp fibroblast-like cells, hDDPF



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DOI: https://doi.org/10.11223/jarde.22.46