Journal of Oral Tissue Engineering

Effects of MC3T3-E1 Cells and Balb c/3T3 Cells for Recovery by Loading In Vitro

Nobuko KAWASHIMA1, Fumitake UENO1, Koichi IMAI2, Tatsuo KAWAMOTO1

1Department.of Orthodontics, Osaka Dental University, 2Department of
Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho,
Hirakata, Osaka 573-1121, Japan

J Oral Tissue Engin 2004;2(1): 35-39

Full Text. DOI

The aim of this study was investigate the influence of the degree of damage suffered by MC3T3-E1 cells, which are osteoblast-like cells derived from the mouse, as compared to the degree of damage incurred by Balb c/3T3 cells. Immediately after loading, the amount of lactate dehydrogenase (LDH) increased in proportion to the load. In four days cultivation, no significant departure from the absorbance of the control group was observed other than in the 40g group, but in the MC3T3-E1 cells the amount of LDH was as great as that immediately after loading, whereas in the Balb c/3T3 cells the amount decreased to about 70%. A comparison of the two types of cells has revealed that the MC3T3-E1 cells, which take part in the bone formation, can withstand slightly less loading than the Balb c/3T3 cells.

Key words:MC3T3-E1 cell, Balb c/3T3 cell, LDH, cell recovery, Invisalign