Journal of Oral Tissue Engineering
In vitro Osteogenic Differentiation Potential of Dental Pulp Stem Cells
Masaharu TAKEYASU1, Tadashige NOZAKI2, Masatoshi WATANABE3,
Mitsuko SHINOHARA2, Joji MORITA4, Atsushi HIDAKA2,
Kazuya IWAMOTO2, Toshifumi TAKAHASHI2, Sachiko NAGATA1,
Michiharu DAITO1, Kiyoshi OHURA2
1Department of Pediatric Dentistry, 2Department of Pharmacology, Osaka Dental
University, 8-1, Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan.
3Laboratory for Medical Engineering, Division of Materials Science and Chemical
Engineering, Graduate School of Engineering, Yokohama National University,
79-5, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
4Second Department of Pathology, Mie University School of Medicine,
2-174, Edobashi, Tsu, Mie 514-8507, Japan.
J Oral Tissue Engin 2004;2(1): 25-30
Full Text. DOI https://doi.org/10.11223/jarde.2.25
In the present study, the in vitro osteogenic differentiating potential of rat dental pulp stem cells was examined. The induction was carried out under the same inducing system as used for bone marrow cells. Alkaline phosphatase (ALP) activity and min-eralization were measured at 1, 2, 3, and 6 weeks after the induction. The level of ALP activity was very high at 1 week after induction and the level was maintained for 3 weeks, however the level of ALP activity was dramatically decreased (to 50%) after 6 weeks. Although mineralization was detected at low levels after 3 weeks, it became expanded to up to 29 nodules mineralized at 6 weeks after induction, as determined by von Kossa staining. It was shown that in vitro osteogenic differentiation of rat dental pulp stem cells was induced following the increase of the ALP activity at an early stage of the induction.
Key words: dental pulp, differentiation, bone