Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Evaluation of Bone Regeneration using Dentinal Granules with Autologous Bone

 Kenichirou YASUI1, Masayasu TATSUMURA1,
Jinzhao LYU1, Yuji KURUSHIMA1, Ming ZHAO1,
Kenji TSUDA1, Wenqi DENG1, Hidetoshi MORIKUNI1,
Aki NISHIURA1, Yoshiya HASHIMOTO2,
and Naoyuki MATSUMOTO1


1Department of Orthodontics, Osaka Dental University, Osaka, Japan
2Department of Biomaterials, Osaka Dental University, Osaka, Japan
J Oral Tissue Engin 2020; 18(1): 13-22.
SYNOPSIS
A bone defect was created at the center of a rat calvaria. Autologous bone and dentinal granules with autologous bone were transplanted. All specimens were evaluated microradiographically and histologically. CT revealed no difference in the amount of hard tissue between the autologous bone and dentinal granules with autologous bone group 4, 6, and 8 weeks post-surgery. Tissue analysis of the dentinal granules with autologous bone group, 8 weeks post-surgery, revealed that the transplanted autologous bone granules were entirely replaced with the new bone. Meanwhile, about a quarter of the added dentinal granules remained as dentinal granules with autologous bone, and the rest were replaced with autologous bone. In addition, the diameter of the dentin granules decreased with time. Therefore, the addition of dentinal granules to autogenous bone grafts does not inhibit replacement of the new bone and imparts bone regeneration ability to autologous bone grafts for a prolonged period of time.

Key words: bone tissue engineering, autologous bone graft, dentin graft, CT analysis, rat calvarial defect

All documents in this paper

J-Stage https://www.jstage.jst.go.jp/article/jarde/18/1/18_13/_article

DOI https://doi.org/10.11223/jarde.18.13