Journal of Oral Tissue Engineering

Effects of Transforming Growth Factor-β1 and Bone Morphogenetic Protein-2 on Bone Formation using a Bioabsorbable Scaffold at Palatal Sites in Rats

Kozo YAMAJI1, Akihito YOKOYAMA1, Yasushi SHIMADA1, Tsutomu SUGAYA2, Naoko OHARA1,
and Masahiro YOSHIYAMA1

1Department of Operative Dentistry, Field of Study of Biofunctional Recovery and Reconstruction,
Okayama University Graduate School of Medicine,
Dentistry and Pharmaceutical Science, Okayama, Japan
2Department of Periodontology and Endodontology,
Division of Oral Health Science, Hokkaido University Graduate School of
Dental Medicine, Sapporo, Japan

J Oral Tissue Engin 2018; 16(2):74-79.

This study was designed to examine the effects of transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) on bone formation using a bioabsorbable scaffold at palatal subperiosteal sites in rats. Alginate gel containing TGF-β1 (0.0 or 0.1µg / µL) and BMP-2 (0.0 or 0.1µg / µL) were used for implantation, and experimental sites were divided into four groups according to the cytokines contained in the scaffold: TGF group (TGF-β1), BMP group (BMP-2), TGF+BMP group (TGF-β1 and BMP-2) and Control group. Four weeks after implantation, thickness of new bone (TNB) was significantly higher in TGF group and BMP group than in Control group (P<0.05). There was no significant difference in TNB between TGF group and BMP group (P>0.05). On the other hand, TNB was significantly higher in TGF+BMP group than in TGF group and BMP group (P<0.05). These results suggest that TGF-β1 and BMP-2 contained in alginate gel have interactive effects on bone formation in the case of palatal subperiosteal sites.

Key words: TGF-β1, BMP-2, bone formation

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