Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Wnt Signal Pathway Regulates MMP-1 and MMP-3 Production in Human Dental Pulp Fibroblast like Cells

 Osamu TAKEUCHI1, Reiko KOMASA1, Yukiko HOSOYAMA2, Kazushi YOSHIKAWA1,
Seiji GODA3, and Kazuyo YAMAMOTO1

1Department of Operative Dentistry, 2Department of Orthodontics,
Osaka Dental University, Osaka, Japan
3Department of Oral Science, Graduate School of Dentistry,
Kanagawa Dental University, Yokosuka, Japan
J Oral Tissue Engin 2018; 16(2):47-56.
SYNOPSIS
Inflammatory cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were previously shown to be secreted in pulpitis tissue during the caries process. Matrix metalloproteinases (MMPs) such as MMP-1, -2, -3, and -14 were also shown to be expressed in inflamed dental pulp. MMP-3 activates other MMPs. MMP-1 and MMP-3 can degrade the extracellular matrix (ECM). Dental pulp destruction may be regulated, in part, by MMP-1, and MMP-3 activated by MMP-3 has been shown to regulate the degradation and regeneration of dental pulp. In this study, we tested an activator and an inhibitor of Wnt/β-catenin signaling to regulate IL-1β and TNF-α -induced production of MMP-1 and MMP-3 in human dental pulp fibroblasts cells (HPFs). We demonstrated that MMP-1 and MMP-3 were produced from HPFs in response to TNF-α in a Wnt/β-catenin signaling -dependent manner. These results suggest that a Wnt/β-catenin signal regulating drug may have utility for dental pulp tissue regeneration.

Key words: MMPs, Dental pulp fibroblasts, Wnt/β-catenin

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J-Stage https://www.jstage.jst.go.jp/article/jarde/16/2/16_16_47/_article/-char/en

DOI https://doi.org/10.11223/jarde.16.47