Journal of Oral Tissue Engineering

Influence of Phototoxicity on 8-MOP by Human Keratinocyte NHEK Cells

Hiroki ISHIKAWA1, Rie IWAI2, Kaname TSUJI2, Yuki MATSUSHIMA2, Aoi FUKUDA2, Koji YAMADA2, Kazuhiro MATSUMOTO2, Hiroaki YOSHIDA2, Tomio ISEKI2, Hideo SHIMIZU2,4, Mamoru UEDA1, Koichi IMAI3, and Shosuke MORITA2

1Graduate School of Dentistry (First Department of Oral Maxillofacial Surgery),
2Department of Oral Maxillofacial Surgery, 3Department of Biomaterials, Osaka Dental University, Osaka, Japan,
4Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Osaka, Japan

J Oral Tissue Engin 2018; 15(3): 171-176.

Phototoxicity, i.e., cytotoxicity caused by light irradiation, is due to the biological effects of active oxygen and free radicals, generated by energy released upon the excitement of chemical substances by ultraviolet irradiation, and those of photo-excited chemical substances. We have already conducted a phototoxicity test on 8-MOP using six cell types, including mouse-derived 3T3 cells and human oral cavity-derived tumor cells, demonstrating potent phototoxicity in oral cancer-derived cells.
Since hyperkeratosis occurs in some oral mucosal diseases as in inflammatory keratosis (psoriasis), a phototoxicity test was conducted with human keratinocytes. As a result, the phototoxicity of 8-MOP was demonstrated in two-dimensional culture, and was more significant in three-dimensional culture.

Key words: phototoxicity, 8-MOP, human keratinocyte, NHEK

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