, cytotoxicity caused by light irradiation, is due to the biological effects of active oxygen and free radicals, generated by energy released upon the excitement of chemical substances by ultraviolet irradiation, and those of photo-excited chemical substances. We have already conducted a phototoxicity test on 8-MOP using six cell types, including mouse-derived 3T3 cells and human oral cavity-derived tumor cells, demonstrating potent phototoxicity in oral cancer-derived cells.
Since hyperkeratosis occurs in some oral mucosal diseases as in inflammatory keratosis (psoriasis), a phototoxicity test was conducted with human keratinocytes. As a result, the phototoxicity of 8-MOP was demonstrated in two-dimensional culture, and was more significant in three-dimensional culture.
Key words: phototoxicity, 8-MOP, human keratinocyte, NHEK
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