Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Study of Differentiation Level by Three-dimensional Culture of Mouse ES Cells on Titanium Alloy Powder

 Koichi IMAI1, Tsubasa SHIRAI1, Mari AKIYAMA1, Masayuki ZENNYU1, Takamitsu YOSHIDA1, Tomoharu OKAMURA2, Muneyasu SHIDA3, Hideo SHIMIZU4,5, Shosuke MORITA4, Kazuya TOMINAGA2, Kazuya MASUNO6, Hidenori MATSUMOTO6, and Tetsunari NISHIKAWA6

1Department of Biomaterials, 2Department of Oral Pathology, 3Department of Endodontics,
4First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,
5Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Osaka, Japan,
6Department of Innovations in Dental Education, Osaka Dental University, Osaka, Japan
J Oral Tissue Engin 2018; 15(3): 159-164.
SYNOPSIS
Pure titanium surface is strongly bound to the bone when embedded as a dental implant, with high biocompatibility to the soft tissue around the implant. However, the mechanical strength of Ti-6Al-4V alloy is superior to that of pure titanium. Titanium alloy products account for about 40% of all implant products in Japan. Of the composition elements of Ti-6Al-4V alloy, vanadium has a higher toxicity than the others. Ti-6Al-4V alloys are employed in various titanium alloy implant products. In the present study, cell differentiation and proliferation levels were examined with a polished Ti-6Al-4V alloy in three-dimensional ES cell culture on collagen, demonstrating no developmental toxicity of Ti-6Al-4V alloy. However, Ti-6Al-4V alloy powder corroded with a hydrofluoric acid solution had effects on the differentiation and proliferation of ES cells.

Key words: Ti-6Al-4V, titanium alloy, cell differentiation, ES cell, cytotoxicity



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