Journal of Oral Tissue Engineering

Evaluation of Tissue Behavior on Three-dimensional Collagen Scaffold Coated with Carbon Nanotubes and β-tricalcium Phosphate Nanoparticles

Hirofumi MIYAJI1, Shusuke MURAKAMI1, Erika NISHIDA1, Tsukasa AKASAKA2, Bunshi FUGETSU3, Junko UMEDA4, Katsuyoshi KONDOH4, Tadashi IIZUKA5, and Tsutomu SUGAYA1

1 Department of Periodontology and Endodontology,
2 Department of Biomedical Materials and Engineering,
Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan
3 Policy Alternatives Research Institute, The University of Tokyo, Tokyo, Japan
4 Joining and Welding Research Institute, Osaka University, Osaka, Japan
5 Support Section for Education and Research, Faculty of Dental Medicine,
Hokkaido University, Sapporo, Japan

J Oral Tissue Engin 2018; 15(3): 123-130.

We generated a three-dimensional collagen scaffold coated with carbon nano-tubes (CNTs) and β-tricalcium phosphate (β-TCP) nanoparticles and histologically evaluated tissue behavior toward the nanomodified scaffold after subcutaneous tissue implantation in rat. Scanning electron microscopy images of the nanomodified scaffold showed that the collagen surface was enveloped by a meshwork of CNTs and dispersed β-TCP nanoparticles. Histological observations indicated that application of CNTs and β-TCP nanoparticles increased cell and blood vessel penetration into the collagen scaffold. CNTs consistently stimulated giant cell aggregation. In addition, CNTs and β-TCP application to the scaffold significantly promoted the DNA content of infiltrating cells and scaffold biodegradation compared to the untreated scaffold. The nanomodified scaffold coated with CNTs and β-TCP nanoparticles would be beneficial for tissue engineering therapy.

Key words: biocompatibility, nanocarbon, rat, tissue engineering

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