SYNOPSISIn this study, two different proteins, fibronectin (FN) or albumin (Alb), were immobilized onto poly (lactic acid-ε-caprolactone) (PLCL) films. FN is a cell adhesion protein, whereas Alb is a cell adhesion-inhibiting protein.The amount of immobi-lized FN on the PLCL film was significantly greater than that for Alb (p < 0.05). The number of MC3T3-E1 cells that adhered to FN-PLCL films was larger than that for Alb-PLCL films (p < 0.05) in a 90-min cell assay. After 3 days of culture, cells appeared more flattened on FN-PLCL films, and small lamellipodia and filopodia were observed. These results indicate that FN and Alb-immobilized onto PLCL films retained their cell adhesion-promoting and cell adhesion-inhibiting properties, respectively.
Key words: Poly (Lactic Acid-ε-Caprolactone) film, Fibronectin, Albumin, Osteoblast-like cell, Alkali treatment
All documents in this paper
J-Stage
https://www.jstage.jst.go.jp/article/jarde/15/1/15_15_3/_article/-char/en
DOI
https://doi.org/10.11223/jarde.15.3