Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Effects of Aging on Bone Formation Induced by BMP-2 using Low-intensity Pulsed Ultrasound

 Kozo YAMAJI1, Akihito YOKOYAMA1, Kumiko MATSUZAKI1, Naoko OHARA1, Eriko NISHIYAMA1, Tsutomu SUGAYA2, and Masahiro YOSHIYAMA1

1Department of Operative Dentistry, Field of Study of Biofunctional Recovery and Reconstruction, Okayama University Graduate School of Medicine,
Dentistry and Pharmaceutical Science, Okayama, Japan
1Department of Peridontology and Endodontology, Division of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Japan
J Oral Tissue Engin 2016; 14(1): 65 - 69.
SYNOPSIS
The purpose of this study was to evaluate the effects of aging on bone formation induced by Bone Morphogenetic Protein-2 (BMP-2) using Low-intensity pulsed ultrasound iLIPUSjin 12-weeks-old (12w group) and 70-weeks-old (70w group) Wister rats.
The experimental sites (palatal subperiosteal sites) were divided into three sites as follows: BMP site, BMP-2 was implanted; LI-BMP site, LIPUS treatment was performed every three days after implantation of BMP-2; and Control site, neither implantation of BMP-2 nor LIPUS treatment was done.
At 3 weeks after surgery, thickness of new bone (TNB) was significantly higher in both the BMP and LI-BMP sites than in the Control site (P<0.05). TNB of every site in the 12w group was significantly higher than that of the same experi-mental site in the 70w group (P<0.05). Newly formed bone was almost integrated with original bone at the LI-BMP site.
These results suggest that LIPUS enhances the maturation of bone formation induced by BMP-2 even in senescent rats, although the volume of bone decreased with aging.

Key words: aging, bone formation, BMP, LIPUS