Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Amlodipine Inhibits the G1/S Cell Cycle Transition Induced by Basic Fibroblast Growth Factor in Human Gingival Fibroblasts

 Reiri TAKEUCHI1, Kazumune ARIKAWA2, Toshikazu UCHIYAMA3, Etsuko AIDA4, Michiko TAKEFUTA5, Koichi NAKAKUKI6 and Tadahiko UTSUNOMIYA7
Departments of 1Pharmacology, 2Community Oral Health, 3Renascent Dentistry, 4Crown Bridge Prosthodontics, 7Oral Pathology,
Nihon University School of Dentistry at Matsudo, Chiba, Japan
5Nihon University Dental Hygiene School, School of Dentistry at Matsudo, Chiba, Japan
6Maxillofacial Surgery, Graduate School of Medical and Dental Sciences,
Tokyo Medical and Dental University, Tokyo, Japan
J Oral Tissue Engin 2013;11(2):141-147
SYNOPSIS
Recently, it has been suggested that topical application of basic fibroblast growth factor (bFGF) is an effective treatment for periodontal tissue regeneration. Gingival fibroblasts (GFs) play an important role in periodontal tissue regeneration. Many patients who receive periodontal therapy take amlodipine (AML). Since AML inhibits cell proliferation via cell cycle arrest, it may hinder the periodontal tissue regeneration induced by bFGF. In this study, we investigated whether AML affects the cell cycle and the expression of its control genes in the presence of bFGF in human GFs (hGFs). Semi-confluent cells were synchronized at the G0/G1 phase in DMEM containing 0.5% serum (DMEM-0.5) for 24 hrs. After pretreatment with or without 3 or 10 μM AML in DMEM-0.5 for 24 hrs, cells were stimulated with 10 ng/mL bFGF in serum-free DMEM. Then, analyses of the cell cycle and the cell number, and RT-PCR analysis were performed. Our results showed that AML inhibited the bFGF-induced G1/S cell cycle transition through suppressing the mRNA expression of Cdk 1, 2, 4, and 6 and cyclins A, D1, and E in hGFs. In conclusion, AML may exert a negative influence on periodontal tissue regeneration induced by bFGF.

Key words: amlodipine, basic fibroblast growth factor, cell cycle, gingival fibroblast, periodontal tissue regeneration