Journal of Oral Tissue Engineering

An Attempt to Study of Mouse ES Cell Differentiation using Collagen Derived from Tilapia Scaley

Koichi IMAI1, Toshimasa UEMURA2, Akito TANOUE3, Kazuaki NAKAMURA3, Kazuhiko SUESE4 and Hiromasa TAKASHIMA5

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan
3Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
4Osaka Dental University, School of Dental Technician and Hygienist, Osaka, Japan
5Ina Research Co.,Ltd., Nagano, Japan

J Oral Tissue Engin 2012;10(2):89-94

Porcine atelocollagen has traditionally been used as a scaffold for cell culture in the field of regenerative medicine. Marine collagen, rather than collagen derived from mammals has recently attracted attention. However, the collagen of fish (e.g., salmon) that inhabits cold oceans has already been demonstrated to be unsuitable as a three-dimensional scaffold for long-term culture at 37°C. In the present study, we examined whether type I collagen, extracted from the scales of tilapia inhabiting tropical and subtropical areas, can be used as a scaffold for ES cells. As a result, it had no effect on ES cell differentiation as compared with conventional porcine collagen. In a previous study, salmon collagen could not withstand long-term culture. Thus, the collagen obtained from tilapia, featuring a high denaturation temperature (35-37°C), is useful as a scaffold for ES cell differentiation.

Key words: ES cells, 3D culture, Tilapia, scafford, collagen