Journal of Oral Tissue Engineering

Fabrication of the Fibronectin-immobilized Three-Dimensional Porous Poly(lactic acid) Scaffold and Initial Mouse Osteoblast-like Cell Attachment

Hirotaka OOMINE1, Megumi FUSE2, Tohru HAYAKAWA3 and Masahiko FUKUMOTO2
1 Department of Maxillo-Facial Orthodontics Medicine,
2Department of Laboratory Medicine for Dentistry,
Nihon University School of Dentistry at Matsudo, Chiba, Japan
3Department of Dental Engineering, Tsurumi University
School of Dental Medicine, Yokohama, Japan

J Oral Tissue Engin 2012;10(2):59-66

In the present study, three-dimensional (3D) porous poly(lactic acid) (PLA) scaffold was fabricated by using progen and freeze-dry method and then fibronectin was immobilized after NaOH treatment on 3D porous scaffold. The biological effect of fibronectin immobilization on PLA scaffold was evaluated by the measurement of initial attachment of MC3T3-E1 osteoblast-like cells and by the morphological observation of attached cells. The number of attached cells onto FN-PLA scaffolds were significantly greater than on PLA scaffolds after 90 min culture (p<0.05). The attached cells were flattened and fastened on FN-PLA scaffold surface and the presence of lamelipodia and flopodia was recognized. Moreover the attached cell could be observed not only the surface of 3D porous PLA scaffold but also the inside of the scaffold by fibronectin immobilization. On the other hand, cells were rounded shape on PLA scaffold surface and there are no formation of lamelipodia and flopodia. The attached cells were not seen in the inside area of PLA scaffold.
In conclusion, it revealed that fibronectin immobilization of 3D porous PLA scaffold was effective for biological activities of osteoblast-like cells.

Key words: three-dimensional porous poly(lactic acid) scaffold, fibronectin, osteoblast-like cell, Alkali treatment