Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
Human Oral Epithelial and Periodontal Ligament Cells Sheets Cultured on Human Amniotic Membrane for Oral Reconstruction

Takeshi AMEMIYA1, Takahiro NAKAMURA2, Fumishige OSEKO1, Toshiro YAMAMOTO1, Atsuo FUKUSHIMA1, Akira NAKANISHI1, Shigeru KINOSHITA2 and Narisato KANAMURA1

1Department of Dental Medicine, 2Ophthalmology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
465, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, 602-8566, Japan

Original Paper:J Oral Tissue Engin 2004;1(1): 89-96

Full Text. DOI https://doi.org/10.11223/jarde.1.89

OBJECTIVE: Using human amniotic membrane (AM), we developed a novel, cultured, mucosal epithelia and periodontal ligament (PDL) cells sheet biomaterials from human oral epithelia and PDL cells for oral reconstruction.
MATERIALS AND METHODS: Oral mucosal epithelia and PDL cells were cultivated on human AM carrier. Cultured human oral epithelia and PDL like cells were examined histomorphologically. RESULTS: After 2-3 weeks in culture, oral mucosal epithelial cells developed five to seven layers of stratified and well-differentiated cells. After 10 days in culture, PDL like cells showed a monolayered structure on AM.
CONCLUSIONS: Oral mucosal epithelia and PDL like cells cultivation using AM enable sheet formation. These findings lead us to conclude that this novel oral epithelial sheet material will be a useful biomaterial for oral reconstruction in humans.
Key words:Cultured oral epithelial sheets; Culture periodontal ligament cell sheets; Human amniotic membrane; Tissue engineering.