Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Immobilization of Fibronectin onto Organic Hexamethyldisiloxane Coatings with Plasma Surface Modification
-Analysis of Fibronectin Adsorption Using Quartz Crystal Microbalance-dissipation Technique-

Masao YOSHINARI1, Tohru HAYAKAWA2, Kenichi MATSUZAKA3, Takashi INOUE3, Yutaka ODA1, Masaki SHIMONO4

1Department of Dental Materials Science and Oral Health Science Center,
Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan
2Department of Dental Materials, Research Institute of Oral Science,
Nihon University School of Dentistry at Matsudo,
2-870-1, Sakaecho-nishi, Matsudo, Chiba 271-8587, Japan
3Department of Clinical Pathophysiology, Oral Health Science Center,
Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan
4Department of Pathology, Oral Health Science Center,
Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan
Original Paper:J Oral Tissue Engin 2004;1(1): 69-79
Full Text. DOI https://doi.org/10.11223/jarde.1.69
The immobilization behavior of fibronectin onto plasma-surface modified titanium was evaluated by using the quartz crystal microbalance-dissipation (QCM-D) tech-nique. The plasma-surface modifications included a thin-film coating of hexame-thyldisiloxane (HMDSO) and an O2-plasma treatment on titanium. The contact angle of HMDSO-coatings against double-distilled water was 100 degrees, and dramati-cally decreased after the O2-plasma treatment with less than 5 degrees. XPS analy-sis indicated the introduction of an O2-functional group onto the HMDSO surface by the O2-plasma treatment. The QCM-D technique enabled evaluation the adsorption behavior of fibronectin. The largest amount of fibronectin adsorbed was on both as-coated HMDSO (hydrophobic surfaces) and O2-plasma treated at 10-min (hy-drophilic surfaces) surfaces compared to O2-plasma treated (24-h) surfaces. These results indicated that two adsorption mechanisms of the fibronectin to substrates were considered, including hydrophobic interaction and ionic bond via OH group and/or O2-functional group concomitant with hydrophilic surfaces.
Key words:Surface modification;Plasma polymerization;Adsorption;Fibronectin;Quartz crystal microbalance