Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
Effects of the Medium Type, Serum Amount and Dexamethasone Induction on the Cell Proliferation and Alkaline Phosphatase Activity of Twice-passaged SD Rats' Bone Marrow Stromal Cells

Masayuki TAIRA1, Naoyuki CHOSA2, Setsuo SAITOH1, Kaori SASAKI1, Nobuko SATO2, Junzo TAKAHASHI3 and Yoshima ARAKI1

1Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry,
1-3-27, Chuo-dori, Morioka, Iwate 020-8505, Japan
2Department of Biochemistry, Iwate Medical University School of Dentistry
1-3-27, Chuo-dori, Morioka Iwate 020-8505, Japan
3Department of Oromaxillofacial Regeneration, Osaka University Graduate School of Dentistry,
1-8 Yamadaoka, Suita, Osaka 565-0871, Japan

Original Paper:J Oral Tissue Engin 2004;1(1): 59-68

Full Text. DOI https://doi.org/10.11223/jarde.1.59

The purpose of this investigation was to examine the effects of medium type (α-MEM or DMEM); serum amount (5, 10 or 15%); and dexamethasone (Dex) induction (Dex (-) or Dex (+)) on the proliferation and alkaline phosphatase (ALP) activity of twice-passaged SD rat's bone marrow stromal cells. (1) After 6 days culture (before confluence), cell number and total protein concentration were measured. It was found that the serum amount played the dominant role in increasing the proliferation. (2) After 24 days culture (long after confluence), total protein concentration and ALP activity were measured. It was found that α-MEM was superior to DMEM in producing larger total protein concen-trations. Incrementing the serum amount tended to the increase in total protein concen-trations. Dex induction (Dex(+)) decreased values of ALP activity / total protein concen-tration, reflecting the advance of the osteogenic differentiation stage. The use of Dex (+)α-MEM medium was preferred to produce greater protein production with higher os-teogenic differentiation level
Key words: Bone marrow stromal cells, Cell proliferation, Osteogenic differentiation