Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent Vol.7(1) pp.47-55
ISSN 1348-9615


Dentin Regeneration by the Differentiated Human Bone Marrow Mesenchymal Stem Cells

Shunji IZAWA, Kozo YAMAJI, Tomohiro HOSHIKA, Yasuo SHINNO, Yoshihiro NISHITANI, Masahiro YOSHIYAMA
Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

Dentin regeneration to the lost dentin is the supreme therapy to keep functionality, aesthetics and so forth in operative dentistry. In 2006, we demonstrated the human bone marrow mesenchymal stem cells (hMSCs) differentiated into odontoblast by the stimulation of Emdogain Gel and TNF-α set. In 2008, we tried to demonstrate odontoblast that differentiated from hMSCs generate dentin under the artificially synthesized appropriate scaffold and molecular signal in vitro. As a result, the differentiated cell tissue tested positive for osteocalcin by fluorescence antibody method and enzyme antibody technique and positive for calcium compounds with the alizarin red S staining. In addition, we detected the dentin-like tissue outside the cell walls by phase contrast microscopy. These results suggested that hMSCs differentiate into odontoblast and generate dentin using Emdogain Gel and TNF-α set and peptide hydrogel.

Keyword: human mesenchymal stem cells, dentin regeneration, Emdogain Gel, TNF-α, peptide hydrogel