Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent Vol.7(1) pp.10-17
Continuous Measurement of Cell Proliferation and Calcification using Scintillator-incorporated Culture Plates
Akihiko HIRAYAMA, Masao YOSHINARI
Division of Oral Implant Research, Oral Health Science Center, Tokyo Dental College
The purpose of this study is to clarify the possibility of continuous measurement on cell proliferation and calcification using a scintillator-incorporated culture plate (Cytostar-T). Mouse osteoblast-like cells (MC3T3-E1) were cultured using culture plates (Cytostar-T) with culture media including two nuclides (3H-thymidine and 45CaCl2), followed by continuous measurements of 3H and 45Ca were performed in the same well for 21 days using a micro-plate scintillation counter. The results showed it was possible to measure both cell number and the amount of calcification simultaneously and continuously. These results indicate that measurement using a scintillator-incorporated culture plate is promising method with improving the efficiency of the cells, the reagents and the materials used in vitro tests.
Keyword: 3H - thymidine, cell proliferation, calcification products, continuous measurement, MC3T3-E1