Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent Vol.7(1) pp.1-9
Attachment of Human Gingival Fibroblasts onto collagen PLA porous scaffold
Megumi FUSE1, Tohru HAYAKAWA2, Masahiro KOZAKI1, Kazuaki ISHIKURA1, Mana ICHIMURA1, Osamu TSUZUKIBASHI1, Akira FUKATSU1, Masahiko FUKUMOTO1, Masaharu MAKIMURA1
1Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo
2Department of Dental Engineering, Tsurumi University School of Dental Medicine, Yokohama, Japan
Recently, degradable materials such as poly lactic acid (PLA) were used as Guided Tissue Regeneration (GTR) membranes and/or Guided bone Regeneration(GBR) membranes in dentistry. However, these membranes did not have any biological function such as the facilitation of cell adhesion. Additionally, they do not have a 3-dimensional porous structure to arrange the cell into the part of the organization loss. In this study, PLA 3-dimensional porous scaffold was fabricated by combination of porogen method and freeze-dry method. PLA porous scaffold surface was hydrolyzed with alkaline solution and collagen which was cell-adhesive protein was immobilized on PLA porous scaffold surface using water-soluble carbodiimide. The initial attachment of human gingival fibroblasts was examined. PLA porous scaffold with collagen immobilized after 1 hr alkaline hydrolysis showed significantly higher amount of initial cell attachment than PLA porous scaffold without collagen immobilization.
Keyword: PLA porous scaffold, collagen, initial attachment, human gingival fibroblast