Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent Vol.6(1) pp. 21-34
ISSN 1348-9615


Effects of Simultaneous Adding with Supplements (Dexamethasone and β-Glycerophosphate) and BMP-2 on Osteogenesis of Human Osteoblasts

Naoto OKUMORI1, Toshiki ISHIKAWA1, Takashi MAETANI1, Takayasu IGARASHI1, Toshio IGARASHI2, Masao YOSHINARI3
1Japan Institute for Advanced Dentistry,
2Department of Oral and Maxillofacial Surgery, Kanagawa Dental College,
3Division of Oral Implants Research & HRC7, Oral Health Science Center, Tokyo Dental College

The present study aimed to clarify the effect of simultaneous adding with supplements and BMP-2 on osteogenesis of osteoblasts. The proliferation and differentiation of human osteoblasts were evaluated on titanium plates with perforated holes that were laid beneath atelocollagen sponges impregnated with rhBMP-2, and adding the supplement (dexamethasone and β-glycerophsphate) to the culture medium. Simultaneous adding did not influence cell proliferation but increased slightly the ALP activity and osteocalcin content. However, the supplement was more effective than BMP-2 on cell differentiation. These results suggested that dexamethasone and β-glycerophsphate play an important role in the osteoblasts response to BMP-2.

Keyword: human osteoblasts, dexamethasone, β-glycerophsphate, BMP-2, osteogenesis