Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent Vol.5(1) pp.3-10
ISSN 1348-9615


Attachment of Human Gingival Fibroblasts onto Protein Immobilized GTR Membrane

Megumi FUSE1, Tohru HAYAKAWA2, Akira FUKATSU1, Osamu TSUZUKIBASHI1, Ryoko ARAI1, Mana ICHIMURA1, Masahiko FUKUMOTO1 and Masaharu MAKIMURA1
1Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo
2Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo

Now, Guided Tissue Regeneration (GTR) membranes which used biodegradable polymer were used in dental clinics. However, these membranes did not have any biological function such as the promotion of cell adhesion. In this study, GTR membrane surface was hydrolyzed with alkaline solution and collagen or fibronectin which was cell-adhesive protein was immobilized on GTR membrane surface using water-soluble carbodiimide. Afterwards, the attachment and proliferation of human gingival fibroblasts was investigated. It revealed that collagen immobilization onto GTR membrane by alkaline hydrolysis for 10min enhanced cell attachment, but the degradation of GTR membrane was occurred. The development of another method for immobilization cell adhesive protein onto GTR membrane instead of alkaline hydrolysis was necessary.

Keyword: human gingival fibroblast, attachment, GTR membrane, collagen, fibronectin