Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent Vol.4(2) pp.109-115
ISSN 1348-9615


Initial Attachment of Human Gingival Fibroblasts Derived from Diabetic Mellitus onto Collagen Coated Titanium

Magumi FUSE1, Tohru HAYAKAWA2, Reiri TAKEUCHI3, Kenichi NEBUKA1, Hiroaki WAKAI1, Masahiko FUKUMOTO1, Masaharu MAKIMURA1
1Department of Laboratory Medicine for Dentistry,
2Department of Dental Biomaterials,
3Department of Oral Molecular Pharmacology, Nihon University School of Dentistry at Matsudo

Recently, diabetic mellitus is dramatically increasing in our country. A diabetic often induces the serious periodontal disease and following tooth lost. Therefore, there is a necessity for considering the cell activity to the implant materials for the diabetic mellitus. In the present study, we examined the initial cell attachment to titanium plate by using the human gingival fibrobrasts derived from diabetic mellitus patients. The influence of type I collagen coating on titanium plate for the enhancement of initial cell attachment was evaluated. As a result, collagen coating to titanium plate was effective for the improvement of initial cell attachment for non-diabetic mellitus. On the contrary, for human gingival cell derived from diabetic mellitus under the high blood glucose condition of HbA1c 9%, collagen coating to titanium plate was not effective for the enhancement of initial cell attachment.

Keyword: diabetes mellitus, human gingival fibroblast, collagen, titanium