We have developed a novel cultured skin model "Vitrolife-Skin^TM" which is composed of two types of collagen sponges and two types of normal human skin cells, fibroblasts and keratinocytes. This cultured skin model has a two-layer system consisting of dermis and epidermis with cornified layers which likes a real skin. We have evaluated skin irritancy and skin corrosivity to alternate to animal testing and developed a novel chemical application procedure using this cultured skin model. Also, we have developed regeneration of full thickness skin defects using this cultured skin model with the aim clinical application. This report reviews our cultured skin model.