Journal of the Japanese Association of Regenerative Dentistry
J Jpn Assoc Regenerative Dent_Vol.2(3)_pp.182-191
ISSN 1348-9615


N-bisphosphonates regulate the proliferation and the mRNA expression on human osteoblast-like cells

Eisuke DOMAE1, Yoshihiro YOSHIKAWA1, Aiko KAMADA2, Masanori UENO2, Junichiro MAEDA2, Seiji GODA2, and Takashi IKEO2
1Graduate School of Dentistry (Biochemistry), 2Biochemistry, Osaka Dental University

Bisphosphonates(BPs), synthetic analogs of pyrophosphate, are potent inhibitors of bone resorption and are being successfully used with increasing frequency in the treatment of osteoporosis. We investigated whether BPs (etidronate, alendronate and risedronate) affect the proliferation and the mRNA expression of type I collagen, osteocalcin and alkalinephosphatase on human osteoblast-like Saos-2 cells. When alendronate and risedronate were co-cultured with Saos-2 cells, proliferations were deceased compared with control and etidoronate. The mRNA expression was analyzed using a reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of mRNA, type I of collagen, osteocalcin and alkalinephosphatase were decreased by treatment with alendronate and risedronate but not etidronate. These results indicate that N-BPs may influence the activity of osteoblasts not but non-N- BPs.