The aim of this study was to investigate the morphology, growth and type I collagen expression of human pulp derived cells on the poly(ethylene-co-vinyl alcohol) (EVA) coated with collagen (+C) film. Cell morphology and growth curve for EVA+C group was similar to that for control group, which was cultured on the normal cell culture dish. However, for EVA group cell shape showed no extension and the growth was down-regulated. There was no difference in expression of type I collagen in the cultured cells among the three groups.
These results suggested that the immobilization of collagen to EVA facilitate an attachment and extension of human pulp derived cells on the EVA film without down regulation of type I collagen.